陳顯軍 教授 博士生導(dǎo)師

上海市新藥設(shè)計(jì)重點(diǎn)實(shí)驗(yàn)室副主任

生物反應(yīng)器工程國家重點(diǎn)實(shí)驗(yàn)室固定成員

光遺傳學(xué)與合成生物學(xué)交叉學(xué)科研究中心核心成員

上海市細(xì)胞代謝光遺傳學(xué)技術(shù)前沿科學(xué)研究基地成員

聯(lián)系方式

電話/傳真：021-64251287

E-mail: xianjunchen@ecust.edu.cn

通訊地址：上海市徐匯區(qū)梅隴路130號(hào)華東理工大學(xué)光遺傳學(xué)與合成生物學(xué)交叉學(xué)科研究中心315室，郵編：200237

個(gè)人簡介

2009年6月本科畢業(yè)于華東理工大學(xué)生物工程學(xué)院生物技術(shù)專業(yè)，2015年12月博士畢業(yè)于華東理工大學(xué)藥學(xué)院化學(xué)生物技術(shù)與工程專業(yè)。2016年1月開始博士后研究，先后任華東理工大學(xué)藥學(xué)院藥物科學(xué)系特聘副研究員、研究員、教授。迄今已在Nature Biotechnology (2019, Cover; 2022), Nature Methods、Nature Chemical Biology (2021; 2024)、Nature Communications（2021; 2023)、Nature Protocols、Cell Research、Cell Discovery、Nucleic Acids Research (2016; 2020; 2024)、Biosensors and Bioelectronics、Journal of Controlled Release (2023; 2024)、Small等期刊發(fā)表SCI論文多篇，編寫英文著作Curr Protoc Chem Biol 1個(gè)章節(jié)。已申請(qǐng)并授權(quán)多項(xiàng)中國和國際PCT發(fā)明專利。主持國家重點(diǎn)研發(fā)計(jì)劃課題/子課題、國家自然科學(xué)基金原創(chuàng)/面上/青年基金、上海市自然科學(xué)基金等多項(xiàng)國家與省部級(jí)基金項(xiàng)目，擔(dān)任中國生物化學(xué)與分子生物學(xué)酶學(xué)專業(yè)分委會(huì)委員兼副秘書長、上海市生物物理學(xué)會(huì)理事會(huì)理事、上海市化學(xué)化工學(xué)會(huì)生物技術(shù)專業(yè)委員會(huì)委員、九三學(xué)社上海市醫(yī)療衛(wèi)生專門委員會(huì)委員，先后入選國家級(jí)高層次青年人才、中國科協(xié)青年人才托舉工程、上海市青年科技啟明星，獲得2024世界生命科學(xué)大學(xué)“生命科學(xué)新星”、上海市生物化學(xué)與分子生物學(xué)學(xué)會(huì)第二屆“青年新銳獎(jiǎng)”、英國皇家化學(xué)會(huì)Faraday Horizon Prize（團(tuán)體）、華東理工大學(xué)青年英才校長獎(jiǎng)、華東理工大學(xué)青年五四獎(jiǎng)?wù)隆?/span>“東岳藥業(yè)”研究生獎(jiǎng)教金特等獎(jiǎng)。

研究方向

發(fā)展針對(duì)DNA、RNA、蛋白質(zhì)等生物大分子的光遺傳學(xué)監(jiān)測與控制技術(shù)，解析它們?cè)诨罴?xì)胞與活體內(nèi)的時(shí)空動(dòng)態(tài)變化規(guī)律，為探索生物大分子功能及調(diào)控機(jī)制提供實(shí)用的創(chuàng)新工具，同時(shí)也為活細(xì)胞與活體生物傳感、即時(shí)診斷甚至實(shí)時(shí)診斷技術(shù)的發(fā)展提供新的思路。  

招生專業(yè)

生物工程專業(yè)：博士生導(dǎo)師/工程博士導(dǎo)師

藥學(xué)專業(yè)：博士生導(dǎo)師/碩士生導(dǎo)師/工程碩士導(dǎo)師

生物與醫(yī)藥工程專業(yè)：工程博士導(dǎo)師

生物學(xué)：碩士生導(dǎo)師 

代表性論文（#第一作者，*通訊作者）

(1) Zhengda Chen^#, Li Jiang^#, Xiaoyu Liu, Yajie Shi, Yang Liu, Ling Jiang, Jiaxin Li, Xinyi Huang, Siyu Zhou, Bingkun Bao, Bibi Zhang, Qiuning Lin, Qi Bian, Yuxin Yin, Fangting Zuo, Ni Su, Yun Tang, Yuzheng Zhao, Yi Yang*, Xianjun Chen*, Linyong Zhu*. NirFAP680: A Highly Bright and Stable Large Stokes Shift Near-Infrared Fluorogen-Activating Protein. Nature Methods, 2026, in press.

(2) Fangting Zuo^#, Ni Su^#, Xin Xie^#, Li Jiang, Mengyue Fang, Yuzheng Zhao, Linyong Zhu, Xianjun Chen*, Yi Yang*, Live-Cell Imaging of RNA Dynamics Using Bright and Stable Fluorescent RNAs. Nature Protocols, 2026, in press.

(3) Haifeng Zheng, Xiyu Liu, Luhui Liu, Jiarui Hu, Xianjun Chen*, Imaging of Endogenous RNA in Live Cells using Sequence-activated Fluorescent RNA probes, Nucleic Acids Research, 2025, 53, gkae1209.

(4) Li Jiang^#, Fangting Zuo^#, Yuanyuan Pan^#, Ruilong Li, Yajie Shi, Xinyi Huang, Dasheng Zhang, Yingping Zhuang, Yuzheng Zhao, Qiuning Lin, Yi Yang, Linyong Zhu*, Xianjun Chen*, Bright and Stable Cyan Fluorescent RNA Enables Multicolour RNA Imaging in Live Escherichia coli, Small, 2025, 21, e2405165. Front Cover.

(5) Ziyan Xu, Yongrui Liu, Fudong Li, Yi Yang, Hong Zhang, Xing Liu, Xin Xie*, Xianjun Chen*, Yunyu Shi*, Liang Zhang*, Phase separation of hnRNPA1 and TERRA regulates telomeric stability, Journal of Molecular Cell Biology, 2024, mjae037

(6) Kaiyi Huang^#, Qianqian Song^#, Mengyue Fang^#, Deqiang Yao,Xin Shen, Xiaochen Xu, Xianjun Chen, Linyong Zhu*, Yi Yang*, Aiming Ren*, Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift, Nature Chemical Biology, 2024, 20, 1453-1460.

(7) Fangting Zuo^#, Li Jiang^#, Ni Su^#, Yaqiang Zhang, Bingkun Bao, Limei Wang, Yajie Shi, Huimin Yang, Xinyi Huang, Ruilong Li, Qingmei Zeng, Zhengda Chen, Qiuning Lin, Yingping Zhuang, Yuzheng Zhao, Xianjun Chen*, Linyong Zhu*, Yi Yang*, Imaging the Dynamics of Messenger RNA in Live Cells with Highly Bright and Stable Green Fluorescent RNA, Nature Chemical Biology, 2024, 20, 1272–1281.

(8) Jiafeng Zou, Rui Sun, MuYe He, You Chen, Yi Cheng, Chuanhe Xia, Ying Ma, Shulei Zheng, Xiuzhi Fu, Zeting Yuan,Minbo Lan, Kaiyan Lou, Xianjun Chen*, Feng Gao*, Sequential rocket-mode bioactivating ticagrelor prodrug nanoplatform combining light-switchable diphtherin transgene system for breast cancer metastasis inhibition, ACS Appl. Mater. Interfaces, 2023, 15, 46.

(9) Renmei Liu^#, Jing Yao^#, Siyu Zhou^#, Jing Yang^#, Yaqiang Zhang, Xiaoyan Yang, Leshi Li, Yunbin Zhang, Yingping Zhuang, Yi Yang*,Xianjun Chen*,Spatiotemporal control of RNA metabolism and CRISPR-Cas functions using engineered photoswitchable RNA-binding proteins, Nature Protocols, 2024, 19, 374–405.

(10) Li Jiang^#, Xin Xie^#, Ni Su^#, Dasheng Zhang^#,Xianjun Chen^# *, Xiaochen Xu^#, Bibi Zhang, Kaiyi Huang, Jingwei Yu, Mengyue Fang, Bingkun Bao, Fangting Zuo, Lipeng Yang, Rui Zhang, Huiwen Li, Xinyi Huang, Zhengda Chen, Qingmei Zeng, Renmei Liu, Qiuning Lin, Yuzheng Zhao, Aiming Ren*, Linyong Zhu*, Yi Yang*,Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells, Nature Methods, 2023, 20, 1563-1572.

(11) Yi Cheng, Jiafeng Zou, Muye He, Xinyu Hou, Hongtao Wang, Jiajun Xu, Zeting Yuan, Minbo Lan, Yi Yang, Xianjun Chen*, Feng Gao*,Spatiotemporally controlled Pseudomonas exotoxin transgene system combined with multifunctional nanoparticles for breast cancer antimetastatic therapy, Journal of Controlled Release, 2024, 367, 167-183.

(12)Dasheng Zhang^#, Zhengda Chen^#, Zengmin Du^#, Bingkun Bao^#, Ni Su^#, Xianjun Chen*, Yihui Ge, Qiuning Lin, Lipeng Yang, Yujie Hua, Shuo Wang, Xin Hua, Fangting Zuo, Ningfeng Li, Renmei Liu, Li Jiang, Chunyan Bao, Yuzheng Zhao, Joseph Loscalzo, Yi Yang* and Linyong Zhu*, Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporter, Cell Discovery,2023, 9, 56.

(13) Mengyue Fang^#, Huiwen Li^#, Xin Xie^#, Hui Wang, Ying Jiang, Tianyu Li, Bibi Zhang, Xin Jiang, Yueyang Cao, Rui Zhang, Dasheng Zhang, Yuzheng Zhao, Linyong Zhu, Xianjun Chen*, Yi Yang*, Imaging intracellular metabolite and protein changes in live mammalian cells with bright fluorescent RNA-based genetically encoded sensors, Biosensors and Bioelectronics, 2023, 235, 115411.

(14) Miaowei Mao^#, Yajie Qian^#, Wenyao Zhang^#, Siyu Zhou, Zefeng Wang, Xianjun Chen*, Yi Yang*, Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction, Nature Communications,2023, 14, 2172.

(15) Yajie Qian^#, Ting Li^#, Siyu Zhou, Xianjun Chen*, Yi Yang*, A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila, ACS Synthetic Biology, 2023, 12, 664-671.

(16) Muye He, Yuji Sun, Yi Cheng, Jie Wang, Miao Zhang, Rui Sun, Xinyu Hou, Jiajun Xu, Hai He, Hongtao Wang, Zeting Yuan, Minbo Lan, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, Spatiotemporally controllable diphtherin transgene system and neoantigen immunotherapy, Journal of Controlled Release, 2023, 355, 538–551.

(17) Renmei Liu^#, Jing Yang^#, Jing Yao, Zhou Zhao, Dr Wei He, Ni Su, Zeyi Zhang, Chenxia Zhang, Zhuo Zhang, Haibo Cai, Linyong Zhu, Yuzheng Zhao, Shu Quan, Xianjun Chen*, Yi Yang*, Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins, Nature Biotechnology, 2022, 40, 779-786.

(18) Kaiyi Huang^#, Xianjun Chen^#, Chunyan Li, Qianqian Song, Huiwen Li, Linyong Zhu*, Yi Yang*, Aiming Ren*, Structure-based investigation of fluorogenic Pepper aptamer, Nature Chemical Biology, 2021, 17, 1289-1295.

(19) Ting Li^#, Xianjun Chen^#, Yajie Qian, Jiawei Shao, Xie Li, Shuning Liu, Linyong Zhu, Yuzheng Zhao, Haifeng Ye* & Yi Yang*, A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice, Nature Communications, 2021, 12, 615.

(20) Xinyu Hou, Chenting Shou, Muye He, Jiajun Xu, Yi Cheng, Zeting Yuan, Minbo Lan, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, A combination of LightOn gene expression system and tumor microenvironment-responsive nanoparticle delivery system for targeted breast cancer therapy, Acta Pharmaceutica Sinica B, 2020, 10, 1741-1753.

(21) Xie Li, Changcheng Zhang, Xiaopei Xu, Jun Miao, Jing Yao, Renmei Liu, Yuzheng Zhao, Xianjun Chen*, Yi Yang*. A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells, Nucleic Acids Research, 2020, 48, e33.

(22) Jiajun Xu, Muye He, Xinyu Hou,Yan Wang,Chenting Shou, Xiaoran Cai, Zeting Yuan, Yu Yin, Minbo Lan, Kaiyan Lou, Yuzheng Zhao, Yi Yang, Xianjun Chen*, Feng Gao*, Safe and Efficacious Diphtheria Toxin-Based Treatment for Melanoma: Combination of a Light-On Gene-Expression System and Nanotechnology, Molecular Pharmaceutics, 2020, 17 (1), 301-315.

(23) Xianjun Chen^#, Dasheng Zhang^#, Ni Su^#, Bingkun Bao, Xin Xie, Fangting Zuo, Lipeng Yang, Hui Wang, Li Jiang, Qiuning Lin, Mengyue Fang, Ningfeng Li, Xin Hua, Zhengda Chen, Chunyan Bao, Jinjin Xu, Wenli Du, Lixin Zhang, Yuzheng Zhao, Linyong Zhu*, Joseph Loscalzo, Yi Yang*, Visualizing RNA Dynamics in Live Cells with Bright and Stable Fluorescent RNAs, Nature Biotechnology, 2019, 37, 1287-1293. Cover.

(24) Xiaopei Xu, Zhaoxia Du, Renmei Liu, Ting Li, Yuzheng Zhao, Xianjun Chen* and Yi Yang*, A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells, ACS Synthetic Biology, 2018, 7, 2045-2053.

(25) Wansheng Sun, Wenyao Zhang, Chao Zhang, Miuwei Mao, Yuzheng Zhao, Xianjun Chen*, and Yi Yang*, Light-induced protein degradation in human-derived cells, Biochemical and Biophysical Research Communications, 2017, 487, 241-246.

(26) Xianjun Chen, Renmei Liu, Zhengcai Ma, Xiaopei Xu., Haoqian Zhang, Jianhe Xu, Qi Ouyang, and Yi Yang*, An extraordinary stringent and sensitive light-switchable gene expression system for bacterial cells, Cell Research, 2016, 26, 854-857.

(27) Xianjun Chen^#, Ting Li^#, Xue Wang, Zengmin Du, Renmei Liu, and Yi Yang*, Synthetic dual-input mammalian genetic circuits enable tunable and stringent transcription control by chemical and light, Nucleic Acids Research, 2016, 44, 2677-2690.

(28) Xianjun Chen, Ting Li, Xue Wang, and Yi Yang*, A light-switchable bidirectional expression module allowing simultaneous regulation of multiple genes, Biochemical and Biophysical Research Communications, 2015, 465, 769-776.

(29) Xue Wang, Xianjun Chen, and Yi Yang*, Spatiotemporal control of gene expression by a light-switchable transgene system, Nature Methods, 2012, 9, 266-269.

觀點(diǎn)綜述（#第一作者，*通訊作者） 

(1) Mengyue Fang^#, Ying Jiang^#, Xianjun Chen*, Yi Yang*. Capabilities and challenges for the use of fluorescent RNAs in RNA dynamics research. Trends in Cell Biology, 2025, 2163, 14.

(2) Fangting Zuo^#, Ziheng Gao^#, Xianjun Chen*, Yi Yang*. Fluorescent RNAs: New opportunities for drug discovery. Trends in Pharmacological Sciences, 2025, S0165-6147(25)00096-3.

(3) Xianjun Chen, Xue Wang, Zengmin Du, Zhengcai Ma, Yi Yang*, Spatiotemporal control of gene expression in mammalian cells and in mice using the LightOn system, Curr Protoc Chem Biol, 2013, 5, 111.

(4) 左方婷、張雅強(qiáng)、楊慧敏、楊弋*、陳顯軍*，熒光RNA及其生物傳感技術(shù)研究進(jìn)展，遺傳，2024, 46, 92.

(5) 劉韌玫、李樂詩、楊小燕、陳顯軍、楊弋*，RNA 轉(zhuǎn)錄后代謝時(shí)空精密控制技術(shù)，合成生物學(xué)，2023, 4, 141.

(6)高子珩、鄒譞、周宜、薛婷婷、陳顯軍*、楊弋*，熒光核酸適配體：核酸酶學(xué)分析新機(jī)遇，生物化學(xué)與生物物理進(jìn)展，2023, 50, 1042.

(7) 陳顯軍、左方婷、謝鑫、方夢悅、楊弋*，細(xì)胞RNA影像解析技術(shù)的研究進(jìn)展，生命科學(xué)，2021, 33, 312.

(8) 陳顯軍、左方婷、楊弋*，光控基因表達(dá)系統(tǒng)，生命科學(xué)，2019, 31, 343.